This reagent is also included with the Quant-iT RiboGreen RNA Assay Kit and comes complete with buffer and RNA. 1 ng/μL to 2000 ng/μL, with an extended upper range to 4000 ng/μL. The superior sensitivity and performance of the Modulus™ Microplate Fluorometer allows for dsDNA detection as. Table 2 summarizes results of the. Quant It Picogreen Dsdna Assay Kit Manual - 114,697 . Unit Size. Before using PicoGreen dye to stain DNA, the concentrated Me 2 SO solution containing PicoGreen dye was diluted 200-fold using 1× TE buffer to prepare 2× PicoGreen working solution. Tweet. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. PicoGreen dsDNA quantitation reagents are. We recommend using Quant-IT Picogreen as a high-sensitivity assay to measure DNA concentration in that range . Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. quant it picogreen dsdna assay kit reagents ( Thermo Fisher ) 86. o cDNA quantification using Quant-iT PicoGreen dsDNA Assay Kit (P7589, Life Technologies) o PCR Cycles used as indicated in NUM_PCR_CYCLES_cDNA and NUM_PCR_CYCLES_Nextera x C1_ADD_Reagents: o Randomer added to Lysis Reaction Mix, (3. 5 ng/ml of dsDNA were detected with minimal effect in the presence of ssDNA, RNA, and free nucleotides, which is comparable with InvitrogenTM Quant-iT™ PicoGreen® dsDNA Reagent. One microgram of DNA was sheared using an M220. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. Rapidly detect as little as 0. Product Type. 1 Warm Quant-iT PicoGreen reagent to room temp in the dark. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Final libraries were quantified using a Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) on a SpectraMax iD3 (Molecular Devices). Table 2 summarizes results of the. • Suitable for use in 96- and 384-well plate formats. Selectively detect dsDNA from a wide variety of sources, and even in presence of. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Quantitation Range. low-range. ZERO BIAS - scores, article reviews, protocol conditions and moreComparison of dsDNA dose-response using the Helixyte Green™ Fluorimetric dsDNA Quantitation Kit *High Sensitivity* (blue) with Quant-iT™ PicoGreen ® dsDNA Assay Kit (red ). Add-and-read format makes measuring low concentrations of dsDNA simple—no dilutions, no extra tubes. Data were expressed as mean ± standard deviation (SD) of n = 3 unless otherwise specified. Touch devices users use touch & swipe gestures. Quantification of cfDNA was performed using a TECAN Safire plate reader (96-well format) in combination with the Quant-iT ™ PicoGreen ™ dsDNA Assay Kit (Molecular Probes, Invitrogen; cat #P11496, lot #1911829) according to the manufacturer’s instructions, with slight modifications. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. This protocol is a simplified and c. The differences in mechanical testing, cell proliferation in PrestoBlue™ and Quant-iT™ Picogreen™ dsDNA Assay were evaluated using t-test and one-way ANOVA, followed by Tukey’s post-test with α = 0. The total amount of DNA expected is in the range of 3–100 ng, with concentrations as low as 0. 5mL. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. 2023-11. Related applications: Nucleic Acid Quantitation. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0. Samples should be free of RNA contamination and the use of the Qiagen Reaction Cleanup Kit (Qiagen) is recommended. 50 pg to 2 µg. P7589) was used to quantify DNA by fluorescence. Using the Quant-iTTM PicoGreen® dsDNA reagent and the recommended assay protocol, researchers can quantitate dsDNA in the presence of equi molar concentrations of ssDNA and RNA with minimal effect on the quantitation results. The DNA amount in each library was quantified with the Quant-iT PicoGreen dsDNA Assay Kit and with the Agilent 2200 TapeStation. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. 1X dsDNA assay (%) 100 17. No. P7589. , QuantIt PicoGreen dsDNA assay). Description. The assay is linear over three orders of magnitude and has little sequence dependence. The kit contains: • PicoGreen dsDNA quantitation reagent, 1 ml, stock solution in DMSO • 20 x TE buffer, 25 ml, 200 mM 製品番号（カタログ番号）： P11495. The Qubit dsDNA BR Assay kit contains a highly selective enhancing reagent specific for quantifying dsDNA using volumes as low as 1 µL in a standard 96-well microplate format. 5 ng/mL 990 10 1000 250 pg/mL 999 1 1000 25 pg/mL 1000 0 1000 Blank Figure 1. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). • Specific for dsDNA in the presence of RNA. Mix by gently vortexing for 5 seconds. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 2 to 2,000 ng/µL, providing a detection range of 4−2,000 ng. Catalog Number. (Left) The fluorescence signal from binding of the Quant-iT PicoGreen dsDNA Reagent to dsDNA is linear over at least four orders of magnitude; the inset shows the results obtained using 0–750 pg/ml calf thymus DNA. Bioz Stars score: 86/100, based on 1 PubMed citations. 125 to 100 ng/mL. 蛍光を用いたDNAの定量法の一つとしてInvitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kit が利用されています。. 99 to $1999. Pico488 allows to quantitate as little as low pg/mL of dsDNA. 20pg/µl for Quant-iT PicoGreen® (1µl of sample in a 200µl assay volume). The Quant-iTTM PicoGreenTM dsDNA Reagent enables researchers to quantitate as little as 250 pg/mL of dsDNA (50 pg dsDNA in a 200 μL assay volume) with a fluorescence. PicoGreen定 量检测方法简单、方便，被多家生物制品厂所选择，成为生物制品残留DNA检测的标准。 PicoGreen与DNA双链结合后才发出的荧光，无DNA不发荧光；所发荧光与DNA浓度成 正比。在2010年《中国药典》中提出，PicoGreen定量DNA的方法检出限约0. 0 fluorometer according to the manufacturer’s protocols; a sample volume of 1 μl was. The QFX is a powerful solution for all fluorometric applications where enhanced specificity or sensitivity are needed. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. The Quantus™ NGS Starter package is cost-effective and easy to incorporate into your laboratory. Rapidly detect as little as 0. Both the Quant-iT High-Sensitivity dsDNA Assay Kit and the Quant-iT Broad-Range dsDNA Assay Kit provide concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. . Mit den Quant-iT Picogreen dsDNA-Assay-Kits und dsDNA-Reagenzien erzielen Sie präzise und präzise dsDNA-Konzentrationsmessungen über einen breiten dynamischen Bereich. In addition to the Quant-iT™ dsDNA High-Sensitivity Assay Kit described here, we also offer the Quant-iT ™ dsDNA Broad-Range Assay Kit (Cat. Breadcrumb. 2– 100 ng Quant-iT 1X dsDNA HS Assay Kit – Quant-iT PicoGreen Reagent 1–200 ng Quant-iT OliGreen ssDNA Reagent and Assay Kit. Picogreen assays are compatible with most microplate readers and fluorometers. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Development and characterization of a novel host cell DNA assay using ultra-sensitive fluorescent nucleic acid stain “PicoGreen. Quant-iT™ PicoGreen® dsDNA Assay Kit From Molecular Probes (Invitrogen) May 22, 2007. Product Type. Lambda DNA contained in the kit was used to create a six-point standard curve from 3. Microplate reader setup for a Quant-iT 1X dsDNA HS Assay. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267dsDNA in the presence of equimolar concentrations of ssDNA and RNA with minimal effect on the quantitative results. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). 710 citations – Kit [P7589] Quant-iT™ PicoGreen™ dsDNA Assay Kit by Thermo Scientific. • Specific for dsDNA in the presence of RNA. in the assay in order to reduce waste and still achieve comparable results. 作成者 LatB Staff 01. Prepare the rest of the standards , to prepare enough working solution to assay 100 samples in 200 µL volumes, add 50 µL Quant-iT ™ RiboGreen RNA Reagent to 9. Approximate fluorescence excitation/emission maxima: 510/527 nm (see Figure 1, page 2) Quant-iT ™ dsDNA Broad-Range Assay Kit Catalog no. assay or add 5 µL Quant-iT ™ RiboGreen ™ RNA Reagent to 9. ITSkabir). Unit Size. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. 5 pg-3 ng dsDNA) which has higher accuracy and sensitivity than PicoGreen® in a 96-well assay format. No. 5 mm in the Eppendorf BioSpectrometer fluorescence, PicoGreen reagent is diluted in dimethylsulfoxide (DMSO) which solidifies at refrigerator temperatures. It requires the most user preparation and calculation, but can be adapted for cuvettes or plates. The PicoGreen reagent was freshly prepared by diluting 15 μL reagent with 2 mL of TE buffer (~ 133X) and stored in the dark for no longer than 2 hours. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). Got it. Rapidly detect as little as 0. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays*Selection Guide for the Qubit and Quant-iT Assay Kits—Table 8. 5 Q32851 15. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™ The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. 5ul of 12uM primer diluted in ClontechPicoGreen is highly linear (R 2 ≥ 0. 0 ml 200x in DMSO b. Skip to main list 美谷分子仪器（上海）有限公司. The assay is highly selective for double- stranded DNA over RNA, and in the. io |. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. 12. We use the Quant-iT™ PicoGreen® dsDNAkit for quantification of both extracted and amplified DNA. Picogreen kinetic assay was based on polymerase activity of SARS nsp12 in complex with nsp7L8, which. • Specific for dsDNA in the presence of RNA. Highly selective for dsDNA over ssDNA or RNA. Perform dsDNA quantification easily and quickly with Quant-iT dsDNA assay kits. The fluorescence spectra of dsDNA stained with PicoGreen when in solution equimolar concentration of RNA was also present ([DNA]/[RNA] = 1, red lines in figure 4) did notObtenga mediciones precisas de la concentración de ADNbc en un amplio rango dinámico con los kits de ensayo de ADNbc y reactivos de ADNbc Quant-iT Picogreen. The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. This assay is designed for use with fluorescence microplate readers. Note that the assay volume is dependent on the instrument used to measure fl uorescence; with a microplate reader and a 96-well microplate, the assay volume is reduced to 200 μL and 2,000 assays are possible. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. The PicoGreen is sticking to the sides of the cuvette or assay plate. Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Unlike many other DNA quantitation methods, PicoGreen is minimally affected by the presence of lipid, protein, carbohydrates and other cellular. 7 Q33232 4 Green features Less waste and use of fewer resources With the Qubit and Quant-iT 1X dsDNA HS and 1X dsDNA BR Assay Kits, instead of supplying the assay reagent and dilution buffer in separate bottles as was done with the. Both the Quant-iT High-Sensitivity dsDNA Assay Kit and. • Suitable for use in 96- and 384-well plate formats. The Qubit dsDNA BR (Broad-Range) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of DNA samples. Picogreen assays are compatible with most microplate readers and fluorometers. 특징: Quanti-iT Picogreen을 이용하면 낮은 농도의 DNA 측정에 유리. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. methods for the quantitation of DNA: the Invitrogen™ Quant-iT™ PicoGreen™ dsDNA assay and the Invitrogen™ Qubit™ dsDNA assays. The Quant-iTTM PicoGreen® dsDNA Reagent is an ultra-sensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in molecular biology procedures. Quant-iT™ PicoGreen® dsDNA Assay KitLife TechnologiesCatalog #P7589 protocols. 1 mL kit. The kit is robust with a mix and read format compatible with 96- and 384-well fluorescence-based microplate readers. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. High tolerance for contaminants. The Qubit 1X dsDNA BR Standards includes standards at two concentrations, 0 and 100 ng/µL. Rapidly detect as little as 0. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. 2. quant it picogreen dsdna quantitation assay kit - by Bioz Stars , 2023-09. Quant-iTTM PicoGreen® Assay Reagent 0. 86100 stars. SYPRO and Coomassie Fluor luminescent and fluorescent protein gel stains—Table 9. Technical note | Quant-iT and Qubit 1X dsDNA assays the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. ZERO BIAS - scores, article reviews, protocol conditions and moreSelectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Picogreen assays are compatible with most microplate readers and fluorometers. Therefore, fluorescence spectroscopy using PicoGreen is more useful than UV spectroscopy for evaluating the template activity of DNA for PCR. This protocol is a simplified and condensed. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Detecte rápidamente tan solo 0,25 pg/ml de ADNbc, incluso en presencia de ADNmc. B–F are steps of the assay plate preparation using an test1. samples as well as standard in the assay itself. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 99. DNA standards (Component C) set of 8, 500 µl each (0, 5, 10, 20, 40, 60, 80, 100 ng/µl) 2. Price from $9. Easy to use UV absorbance alternative assay that can be. 2. 0 ml) of the aqueous working solution of Quant-iT™ PicoGreen dsDNA Reagent was added to each cell sample, which was incubated for 10 min at 37 °C in the dark. Picogreen assays are compatible with most microplate readers and fluorometers. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. For Research Use Only. ru by: 1. Sample cDNA concentration was measured using the Quant-iT PicoGreen dsDNA assay kit and black Nunc F96 MicroWell polystyrene plates (ThermoFisher Scientific) according to the manufacturer’s. pDNA encapsulation efficiency of LNP 10 and LNP 20 was measured by using a Quant-iT Pico-Green dsDNA assay kit (ThermoFisher Scientific, Waltham, MA, USA). For detection of picogram levels of DNA on a microplate reader, we recommend our AccuBlue® NextGen dsDNA Quantitation Kit (detection range 2. The Quant-iT™ dsDNA BR assay can be adapted for use in cuvettes or 384-well microplates. Quant-iT ™ PicoGreen ™ dsDNA Assay Kit 1 mL assay kitQuant-iT™ PicoGreen® dsDNA Reagent and Kits | 4 Experimental Protoco l DNA Standard Curve 1. Picogreen assays are compatible with most microplate readers and fluorometers. For highly selective detection of dsDNA over RNA, even in the presence of contaminants. Total amount of 1X TE per assay will be 200 μl per well which includes the amount of TE used to dilute the PicoGreen reagent. Rapidly detect as little as 0. Briefly, 2 µL of sample was diluted in 498 µL TE buffer. Spectral Data: Quant-iT™ PicoGreen® dsDNA reagent 502/523 nm, bound to nucleic acids Quant-iT™ PicoGreen ® dsDNA Reagent and KitsQuant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). PicoGreen dsDNA quantitation reagents are. Dilute from 20xTE stock (supplied in the PicoGreen kit). Similar. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. dsDNA and PicoGreen analyzed using the Modulus and the Blue Fluorescence Optical Kit. Quant It. The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. The Quant-iT family of assay kits—and the related Invitrogen Qubit family of assay kits designed for use with the Invitrogen Qubit 2. Cloning, expression and proteins purification. Picogreen assays are compatible with most microplate readers and fluorometers. Sample fluorescence was. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Thermo Fisher quant it picogreen dsdnaassay kit Quant It Picogreen Dsdnaassay Kit, supplied by Thermo Fisher, used in various techniques. High tolerance for contaminants. BR reagent (Component A) 1. You will need 100 ul diluted PicoGreen per well containing 100 ul sample. Within two years of follow-up, the primary endpoint defined as nonfatal myocardial infarction, stroke or transient ischemic attack, cardiovascular death, and >80% target vessel restenosis occurred in 34 patients (43%). Rapidly detect as little as 0. 2–100 ng of DNA. Figure 1. Briefly, 2 µL of sample was diluted in 498 µL TE. 2000 ng/mL of lamda dsDNA was serially diluted in 1XTE before the addition of the PicoGreen working solution. (Left) The Quant-iT dsDNA Assay Kit, High Sensitivity has a linear detection range of 0. Rapidly detect as little as 0. Abstract. 适用于（设备）. PicoGreen dsDNA quantitation reagents are. The DeNovix dsDNA High. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. • Suitable for use in 96- and 384-well plate formats. 2 to 100 ng) Assay Kit were used with a Qubit 3. Rapidly detect as little as 0. In addition to the Quant-iT™ dsDNA High-Sensitivity Assay Kit described here, we also offer the Quant-iT ™ dsDNA Broad-Range Assay Kit (Cat. for points 1–4 above) prior to cfDNA quantification can be determined for each quantification method and for a range of experimental scenarios for. Sequencing was performed with a HiSeq Single-End Illumina platform (USA) [Citation 31]. The DNA sample used for aliquoting was extracted from soil using the DNeasy PowerSoil Kit (Qiagen). 0 mL to 200 uL to fit a 96-well microplate format. Libraries were sequenced on the Illumina NovaSeq. Add 5uL of PicoGreen reagent for every 1mL of buffer. クイックオーダー. Catalog Number. In addition, the concentration of DNA obtained from the 1860 tomato samples was measured fluorometrically using Quant-iT™ PicoGreen™ dsDNA Assay Kit (hereafter PicoGreen, Thermo Scientific, Waltham, MA, USA) and a 96-wells plate reader VICTOR3 1420 (PerkinElmer, Waltham, MA, USA) equipped with an excitation filter F485 and. HepG2 cell number standard curve determined using the PicoGreen® assay (Quant-iTTM PicoGreen® dsDNA reagent kit, Invitrogen), HepG2 cells were harvested from conventional 2D T75 culture flasks and counted using a haemocytometer and Trypan Blue dye exclusion. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 20–1,000 ng Qubit RNA BR Assay Kit 5–100 ng Qubit RNA HS Assay Kit Varioskan LUX Multimode Microplate Reader 2–1,000 ng Quant-iT dsDNA BR Assay Kit 0. to each well. Quantitation Range. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Quant-iT PicoGreen dsDNA Assay Kit a. PicoGreen reagent provides rapid and easy determination of dsDNA, offering a wide dynamic range. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 50 pg to 2 µg. Specifications Documents Scientific Resources Application Notes Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. Equal amounts (100 µL) of working PicoGreen quantitation reagent were mixed and incubated for 10 minutes at room temperature, protected from light. PicoGreen assay. Samples were excited at 480 nm and the fluorescence was measured at 520 nm using a TECAN Spark multimode reader. The high sensitivity kit is superior to the PicoGreen™ or Quant-iT™ dsDNA detection kits by having aPicogreen是一种极为灵敏的荧光核酸染料，其仅在与DNA双链结合后才发出荧光，且所产生的荧光与DNA浓度呈正比，因而可用于精确测量多个来源的dsDNA，包括基因组DNA、病毒DNA、PCR扩增产物、测序文库等。 本产品Picogreen dsDNA Quantitation Reagent定量具有以下优势：①灵敏度高：可检测低至200 pg的dsDNA，节省. The kit contains: • PicoGreen dsDNA quantitation reagent, 1 ml, stock solution in DMSO • 20 x TE buffer, 25 ml, 200 mM製品番号（カタログ番号）： P11495. Sequencing libraries were built using Illumina's TruSeq Nano DNA LT Library Prep Kit, and the NovaSeq 6000 SP Reagent Kit (500 cycles) was used. a)Use 110 sample number per plate. Quant-iT PicoGreen dsDNA Assay Kit and Reagent (Ex/Em = 502/523 nm) dsDNA: 0. The x-axis gives the mass of nucleic acid when DNA or RNA is assayed alone; in the 1:1 mixture, the total mass of nucleic acid is double. The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. Figure 2. The four reactions per sample were pooled, cleaned up with 1x AMPureXP beads, resuspended in 30 ul EB (10 mM Tris-HCl, pH 8. A Qubit dsDNA BR (broad range, 2 to 1000 ng) Assay Kit and Qubit dsDNA HS (high sensitivity, 0. Picogreen assays are compatible with most microplate readers and fluorometers. BR reagent (Component A) 1. PicoGreen Solution must be diluted fresh before each assay run. Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. The fluorescence intensity as a function of the amount of dsDNA is measured at the optimal. 005% Triton X-100, Tween-20, or Brij. Qubit DNA and RNA assays are available with different quantification ranges. (2012). Spectral Data: Quant-iT™ PicoGreen® dsDNA reagent 502/523 nm, bound to nucleic acids Quant-iT™ PicoGreen ® dsDNA Reagent and KitsQuant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. Quant-iT PicoGreen Reagent Final DNA Concentration in Quant-iT PicoGreen Assay 0 1000 25 ng/mL 900 100 1000 2. Per Well Volume 150 uL/well. Regulations & Guidance; Manuals 0 0. g. PicoGreen dsDNA quantitation reagents are. This protocol is a simplified and This protocol is a simplified and condensed version of the full protocol from the manufacturer. Add µL of the Quant-iT™ PicoGreen® dsDNA Reagent (detailed in Section ) to each sample. Quant-iT™ PicoGreen® dsDNA Assay Kit *10 x 100 µl* 1 kit P11496 Quant-iT™ PicoGreen® dsDNA Reagent 1 kit P7581 Quant-iT™ PicoGreen® dsDNA Reagent *10 x 100 µl* 1 kit P11495 Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 1 kit R11491 Quant-iT™ RiboGreen® RNA Assay Kit *2,000 assays* 1 kit R11490 Quant-iT™ OliGreen™ ssDNA. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The assay is highly selective for double-stranded DNA (dsDNA) over RNA (Figure 1, page 7) and is accurate for initial sample concentrations from 10 pg/µL to 100 ng/µL. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. All cfDNA were quantified by QUBIT dsDNA HS Assay kit (Thermo Fisher Scientific®, Aalst, Belgium), according to the manufacturer’s data. DNA samples with a concentration determined by 260 nm absorbance higher than 100 ng/mL were diluted. Analysis of cfDNA and H3Cit was performed using Quant-iT™ PicoGreen ® dsDNA Assay Kit or an ELISA, respectively. dsDNA만을 선택적으로 정량. You will need 100 μl diluted PicoGreen reagent per well. Qubit® dsDNA BR Assay Kit. assay. Product Type. stranded DNA (dsDNA). Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. 99 to $1999. Working PicoGreen reagent was prepared by diluting the concentrated DMSO-PicoGreen stock solution, provided in the PicoGreen kit, 1:200 with TE according to the kit instructions. 実験してみた – PicoGreenをプレートリーダーで使用してみた. Picogreen assay kit (Invitrogen, P7589) a. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. Quant-iT™ PicoGreen® dsDNA Assay Kit *10 x 100 µl* 1 kit P11496 Quant-iT™ PicoGreen® dsDNA Reagent 1 kit P7581 Quant-iT™ PicoGreen® dsDNA Reagent *10 x 100 µl* 1 kit P11495 Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 1 kit R11491 Quant-iT™ RiboGreen® RNA Assay Kit *2,000 assays* 1 kit R11490 Quant-iT™ OliGreen™ ssDNA. The range of linearity for DNA concentration measurements. If your are using plastic cuvettes or assay plates, reduce this problem by including 0. PicoGreen reagent assays provide a rapid and simple fluorescence-based method for the accurate quantitation of dsDNA. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo. 1–100 ng: Ex / Em (nm) 502 / 523: 510 / 527: 502 / 523: Components: dsDNA HS fluorescent reagent, dilution buffer and standards RCA products may be directly quantified using the Quant-iT® PicoGreen® dsDNA Assay Kit or with a Qubit® Fluorometer after dilution to the appropriate detection range. for points 1–4 above) prior to cfDNA quantification can be determined for each quantification method and for a range of experimental scenarios for each cell line of interest. DNA standards were prepared from concentrated stock herring sperm dsDNA (Sigma, PN-A3294) in TE buffer (tris-EDTA, pH 7. 5 µL of each extracted sample was used for Picogreen analysis. . 8 Measurement of growth factor content in. This kit contains. no. 2 – 100 ng. Catalog Number. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. PicoGreen®試薬アッセイは、蛍光を基にした迅速かつ簡単な手法により、dsDNAを正確に定量することができます。. Picogreen assays are compatible with most microplate readers and fluorometers. Picogreen Assay for DNA quantification: Which one is better to go for, a fresh. Rapidly detect as little as 0. Description. The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. 500/525. 1. Qubit assays are available for double- and single-stranded DNA (dsDNA and ssDNA), RNA, microRNA, and protein. The assay is linear over three orders of magnitude and has little sequence. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. RCA products may be directly quantified using the Quant-iT® PicoGreen® dsDNA Assay Kit or with a Qubit® Fluorometer after dilution to the appropriate detection range. ZERO BIAS - scores, article reviews, protocol conditions and moreGot it. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. 2–100 ng: 2000: O11492 O7582: Quant-iT OliGreen ssDNA Assay Kit and Reagent (Ex/Em = 498/518 nm) ssDNA: 1–200 ng: 2000: R11490 R11491: Quant-iT RiboGreen RNA Assay Kit and Reagent (Ex/Em = 500/525 nm) RNA: 20–1000 ng: 2000: Q32883:After completion of DNA extraction, double-stranded DNA (dsDNA) is quantified by the Quant-iT™ PicoGreen® fluorescence assay using the provided lambda DNA standards (Invitrogen) prior to Library Construction (LC). PicoGreen dsDNA quantitation reagent is a highly sensitive fluorescent nucleic acid stain which is used to quantitate double-stranded DNA. 25 pg/uL 的 dsDNA。 该定量试剂盒在三个数量级内呈线性，几乎无序列依赖性，可让您准确测定多种来源的 DNA，包括基因组 DNA、病毒 DNA、小提 DNA 或 PCR 扩. Picogreen assays are compatible with most microplate readers and fluorometers. The use of a serial dilution of dsDNA standards whileThe quantity of ChIP DNA should first be determined with the Quant-iT™ PicoGreen dsDNA Reagent Kit (Invitrogen). 05 to consider statistical significance. Application observe demonstrating so with Molecular Devices SpectraMax® microplate book and the Quant-iT PicoGreen assay, users can reliably size concentrations as low as 50 pg/mL of double-stranded DNA. Note that the assay volume is dependent on the instrument used to measure fl uorescence; with a microplate reader and a 96-well microplate, the assay volume is reduced to 200 μL and 2,000 assays are possible. • Specific for dsDNA in the presence of RNA. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™ Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. 10 x 100 µL. • Suitable for use in 96- and 384-well plate formats. Each sample was amplified in triplicate, and the triplicate amplicons were pooled and purified by gel extraction and quantified using Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, P7589). The superior sensitivity and performance of the Modulus™ Microplate Fluorometer allows for dsDNA detection as low as 250ng/mL. • Suitable for use in 96- and 384-well plate formats. 86. Both fluorescent assays were performed with a Qubit fluorometer. P11496). Average 86 stars, based on 1. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). Materials: 1. The Quant-iT PicoGreen dsDNA Assay Kit from Thermo Fisher Scientific is more specific for DNA and is about 1000 times more sensitive than traditional absorbance methods. (D–G) TLR stimulation was measured using a HEK293-TLR reporter. Mix PicoGreen and DNA by pippetting up and down 5-10 times. (An additional 1 ml TE with 5 ul PicoGreen may need to be prepared for the standards. PicoGreen dsDNA quantitation reagents are. • Specific for dsDNA in the presence of RNA. 微孔板读数仪. Product Type. Catalog Number. Technical note | Quant-iT and Qubit 1X dsDNA assaysthe Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. quant it picogreen dsdna assay kit reagents ( Thermo Fisher ) 86. 激发/发射. Antibodies Recombinant antibodies, monoclonal & polyclonal antibodies and primary & secondary antibodies. The user guide provides detailed information on the product description, component concentration, storage, assay protocol, and safety data sheets of the Quant-iTTM PicoGreenTM dsDNA Reagent and Kit. Quant-iT PicoGreen dsDNA Assay Kit 를 사용하면, ssDNA 와 RNA 그리고 free nucleotide 가 혼재되어 있는 시료에서도 dsDNA 만을 선택적으로 측정할 수 있으며, 25 pg /ml 의 매우 적은 양까지도 검출할 수 있습니다. g. pDNA encapsulation efficiency of LNP 10 and LNP 20 was measured by using a Quant-iT Pico-Green dsDNA assay kit (ThermoFisher Scientific,. Quant-iT PicoGreen dsDNA Assay Kit (1mL) Quant-iT PicoGreen dsDNA Reagent (1mL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P7589 P7581: Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen. Rapidly detect as little as 0. dsDNA • Quant-iT PicoGreen dsDNA Assay Kit (Cat. Each Helixyte™ assay kit is specially formulated with a target-selective DNA. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Prepare the assay. 2. . PicoGreen dsDNA quantitation reagents are. A 1:200 dilution of PicoGreen reagent is prepared by adding 10 μl of PicoGreen per 2 mL of 1X TE buffer. Silke Angersbach, Ron Earp, Mark Gröne, Franka Maurer, BMG LABTECH, 12/2014. Quant-iT™ PicoGreen ® dsDNA Assay Kit. Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Picogreen assays are compatible with most microplate readers and fluorometers. ZERO BIAS . 0, 20X c. Los ensayos Picogreen son compatibles con la mayoría de los lectores de microplacas y fluorómetros. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. If you would like to use this kit with the Qubit® fluorometer, we have includedconjunction with Molecular Probes' PicoGreen dsDNA Quantitation Reagent enables researchers to quantitate as little as 1 ng/mL of dsDNA. 3,201 3. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA.